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  • Title: [Establishment and application of fetal rolling palate organ culture model of mouse].
    Author: Han J, Xiao Y, Lin JX, Zhang ZF, Li Y.
    Journal: Wei Sheng Yan Jiu; 2006 Jan; 35(1):33-5. PubMed ID: 16598928.
    Abstract:
    OBJECTIVE: To investigate the effect of all-trans retinoic acid (atRA) on the development of mouse palates and its possible mechanism, a model of fetal mouse rolling plate organ culture was establishted. METHODS: Mouse embryonic palates were explanted on GD12 and cultured in a roller device for 72h induced by atRA in different concentrations from 10(-5) micromol/L to 10(-1) micromol/L. RESULTS: Similar to development in vivo, palates in control normal fused. Compared with the control, palates development and fusion were promoted in group of 10(-15) micromol/L, while were inhibited in groups of 10(-4) micromol/L or greater, resulting in cleft palate. The proportion of fusion was reduced in a significant dose-response pattern. CONCLUSION: In the model, the cultured palates continued developing and fusing. Palatal fusion was promoted by normally atRA in 10(-5) micromol/L level and were inhibited by atRA in 10(-4) micromol/L level or greater, which resulting in cleft palate. These mentioned confirm the validity and reliability of this model.
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