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  • Title: [Expression of human LNalpha4LG4-5 in Pichia yeast].
    Author: Lian JQ, Dai XF, Li XH, He FT.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2006 Mar; 22(2):226-30. PubMed ID: 16607948.
    Abstract:
    To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
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