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  • Title: [The application of co-culture system on the in vitro development of bovine somatic nuclear transferred embryos].
    Author: Wu YH, An ZX, Zhang Y, Li XC, He YL, Liu FJ.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2006 Mar; 22(2):306-10. PubMed ID: 16607961.
    Abstract:
    To establish a co-culture system of nuclear transferred embryos in bovine, effects of co-culture cell types, passages and cryopreservation as well as addition of BFF or FBS were investigated. The results showed that embryos co-cultured with oviductal epithelial cell and granulosa cell achieved significantly higher blastocyst rate compared with the control group (P < 0.05) and co-cultured with oviductal epithelial cell had more embryo cell number than those with granulosa cell. Passages of co-culture cells significantly affected the blastocyst rate and embryo cell number (P < 0.05), and cryopreservation decreased the blastocyst rate and embryo cell number remarkably. Supplemention of BFF increased blastocyste rate significantly (P < 0.05). In conclusion, co-cultured with fresh primary oviductal epithelial cell along with addition of 10% BFF in SOFaa could improve development of nuclear transferred bovine embryo in vitro.
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