These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The expression of maxiK channel alpha--subunit during human macrophages differentiating into foam cells.
    Author: Lei XJ, Ma AQ, Xi YT, Zhang W, Yao Y, Du Y, Wang TZ, Geng T.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2006 Mar; 37(2):171-5. PubMed ID: 16608067.
    Abstract:
    OBJECTIVE: To investigate the expression of MaxiK channel alpha-subunit during human monocyte-derived macrophages differentiating into foam cells. METHODS: Human peripheral blood monocytes were isolated from male healthy volunteers by density gradient centrifugation, which, by culture, differentiated further into macrophages as a homogeneous monocyte population. The foam cell model originated from human macrophage was established by incubating macrophages with oxidized low density lipoprotein (OxLDL). The expression of MaxiK channel alpha-subunit was investigated by RT-PCR techniques, Western blotting and immunocytochemistry. RESULTS: After incubating macrophages with 30 mg/L OxLDL for 60 hours, the cellular contents of total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) were markedly increased and the ratio of CE/TC was further raised from (14.437 +/- 6.781) % to (57.946 +/- 3.507) %. Although the expression of MaxiK channel alpha-subunit was downregulated during human monocyte-derived macrophages differentiating into foam cells, there was no significant difference between macrophages and foam cells (P > 0.05). CONCLUSION: That 30 mg/L OxLDL can lead the monocyte-derived macrophage cultured for 60 hours to differentiate into foam cell, but the expression of MaxiK channel alpha-subunit does not change obviously.
    [Abstract] [Full Text] [Related] [New Search]