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  • Title: A novel ribonuclease from fresh fruiting bodies of the portabella mushroom Agaricus bisporus.
    Author: Wang HX, Ng TB.
    Journal: Biochem Cell Biol; 2006 Apr; 84(2):178-83. PubMed ID: 16609698.
    Abstract:
    A 14 kDa ribonuclease with a novel N-terminal sequence was isolated from fresh fruiting bodies of the portabella mushroom. It was adsorbed on DEAE-cellulose and carboxymethyl-cellulose, and demonstrated the highest ribonucleolytic potency toward poly (A), 60% as much activity toward poly (C), 40% as much activity toward poly (U), and the least activity (7% as much) toward poly (G). It exhibited a pH optimum at pH 4.5 and a temperature optimum at 60 degrees C. Its activity at 100 degrees C was higher than that at 20 degrees C.
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