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  • Title: Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection.
    Author: Gao F, Dong J, Li W, Wang T, Liao J, Liao Y, Liu H.
    Journal: J Chromatogr A; 2006 Oct 20; 1130(2):259-64. PubMed ID: 16620855.
    Abstract:
    A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined.
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