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  • Title: Registration of T-2 mycotoxin with total internal reflection ellipsometry and QCM impedance methods.
    Author: Nabok AV, Tsargorodskaya A, Holloway A, Starodub NF, Gojster O.
    Journal: Biosens Bioelectron; 2007 Jan 15; 22(6):885-90. PubMed ID: 16624545.
    Abstract:
    A sensitive optical method of total internal reflection ellipsometry (TIRE) in conjunction with immune assay approach was exploited for the registration of T-2 mycotoxin in a wide range of concentrations from 100 microg/ml down to 0.15 ng/ml. Association constants of 1.4x10(6) and 1.9x10(7)mol(-1)s for poly- and monoclonal T-2 antibodies, respectively, were evaluated from TIRE kinetic measurements. According to TIRE data fitting, binding of T-2 molecules to antibodies (at saturation) has resulted in the increase in adsorbed layer thickness of 4-5 nm. The QCM impedance measurements data showed anomalously large mass increase and film softening, most likely, due to the binding of large T-2 aggregates to antibodies.
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