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  • Title: Phosphorylation-activated 6-phosphofructo-2-kinase from mantle tissue of marine mussels.
    Author: Vázquez-Illanes MD, Ramos-Martínez JI.
    Journal: FEBS Lett; 1991 Dec 16; 295(1-3):176-8. PubMed ID: 1662644.
    Abstract:
    PKF-2 from mussel mantle was phosphorylated by cAMP-dependent protein kinase. The phosphorylation does not change the enzyme activity at neutral pH values, but at acid pH the activity of the phosphorylated form is higher than the native PFK-2. With respect to the native enzyme, the activation consisted of a reduction in the Km for Fru-6-P and a decrease in the inhibitory effect of PEP. These results are in keeping with the stabilized concentration of Fru-2,6-P2 found in the mussel mantle during the physiological hypoxia caused by the closure of the valves.
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