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  • Title: SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections.
    Author: Gomes-Ruiz AC, Nascimento RT, de Paula SO, Lopes da Fonseca BA.
    Journal: J Med Virol; 2006 Jun; 78(6):760-3. PubMed ID: 16628591.
    Abstract:
    Dengue is the most important arthropod-borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real-time polymerase chain reaction may improve diagnosis. TaqMan and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV-3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV-3 was detected and quantitated in 17 IgM-positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV-3, and that for diagnostic purposes, there is no difference between these two assays.
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