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  • Title: Factors derived from mouse embryonic stem cells promote self-renewal of goat embryonic stem-like cells.
    Author: Tian HB, Wang H, Sha HY, Xu XJ, Zhu M, Wu YB, Cheng SH, Chen JQ, Shi YX, Bai ZL, Cheng GX.
    Journal: Cell Biol Int; 2006 May; 30(5):452-8. PubMed ID: 16631391.
    Abstract:
    Goat embryonic stem (ES)-like cells could be isolated from primary materials-inner cell masses (ICMs) and remain undifferentiated for eight passages in a new culture system containing mouse ES cell conditioned medium (ESCCM) and on a feeder layer of mouse embryo fibroblasts (MEFs). However, when cultured in medium without mouse ESCCM, goat ES-like cells could not survive for more than three passages. In addition, no ES-like cells could be obtained when ICMs were cultured on goat embryo fibroblasts or the primary materials-whole goat blastocysts were cultured on MEFs. Goat ES-like cells isolated from ICMs had a normal karyotype and highly expressed alkaline phosphatase. Multiple differentiation potency of the ES-like cells was confirmed by differentiation into neural cells and fibroblast-like cells in vitro. These results suggest that mouse ES cells might secrete factors playing important roles in promoting goat ES-like cells' self-renewal, moreover, the feeder layers and primary materials could also influence the successful isolation of goat ES-like cells.
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