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  • Title: Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy.
    Author: Ganikhanov F, Carrasco S, Sunney Xie X, Katz M, Seitz W, Kopf D.
    Journal: Opt Lett; 2006 May 01; 31(9):1292-4. PubMed ID: 16642089.
    Abstract:
    The signal and idler beams from a picosecond, synchronously pumped optical parametric oscillator (OPO) provide the two colors necessary for coherent anti-Stokes Raman scattering (CARS) microscopy. The OPO provides a continuously tunable frequency difference between the two beams over a broad range of Raman shifts (100-3700 cm(-1)) by varying the temperature of a single nonlinear crystal. The near-infrared output (900-1300 nm) allows for deep penetration into thick samples and reduced nonlinear photodamage. Applications of this light source to in vivo cell and ex vivo tissue imaging are demonstrated.
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