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  • Title: Studies of the Uptake of Nitrate in Barley: I. Kinetics of NO(3) Influx.
    Author: Siddiqi MY, Glass AD, Ruth TJ, Rufty TW.
    Journal: Plant Physiol; 1990 Aug; 93(4):1426-32. PubMed ID: 16667635.
    Abstract:
    (13)NO(3) (-) was used to investigate patterns of NO(3) (-) influx into roots of barley plants (Hordeum vulgare L. cv Klondike) previously grown with (;induced') or without (;uninduced') a source of external NO(3) (-) ([NO(3) (-)](0)). In both induced and uninduced plants, (13)NO(3) (-) influx was biphasic in the range from 0.005 to 50 moles per cubic meter [NO(3) (-)](0). In the low concentration range (<1 mole per cubic meter for induced plants and <0.3 mole per cubic meter for uninduced plants), influx was saturable and V(max) and K(m) values for influx either increased or decreased according to NO(3) (-) pretreatment. By contrast, (13)NO(3) (-) influx in the high concentration range revealed a strictly linear concentration dependence. These fluxes appeared to be mediated by a constitutive, rather than an inducible, transport system.
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