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  • Title: Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis.
    Author: Crété N, Delabar JM, Sinet PM, Créau-Goldberg N.
    Journal: Biotechniques; 1991 Dec; 11(6):711-2, 714, 716 passim. PubMed ID: 1667081.
    Abstract:
    Construction of long-range genomic maps by pulse field gel electrophoresis requires optimum resolution of large DNA fragments. Using the transverse-alternating field electrophoresis system, we describe a method to accurately evaluate the sizes of fragments generated by rare-cutter digestions within the 30-4700-kb range. A protocol generating large (greater than 1000 kb) molecules by partial digestion is also reported.
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