These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transformation of maize via Agrobacterium tumefaciens using a binary co-integrate vector system.
    Author: Zhao ZY, Ranch J.
    Journal: Methods Mol Biol; 2006; 318():315-23. PubMed ID: 16673926.
    Abstract:
    This chapter describes a stepwise protocol to achieve success in genetic transformation of maize using Agrobacterium tumefaciens as a DNA delivery system. Researchers will be able to effectively transform immature embryos of Hi-II and related genotypes with this protocol. The outcome of the transformation process will be transgenic embryogenic callus tissue, transgenic plants, and transgenic progeny seeds. Recommendations for molecular confirmation and evaluation of transgenic tissue/plants are also provided.
    [Abstract] [Full Text] [Related] [New Search]