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  • Title: [An experimental study on the antitumor effects induced by fusions of dendritic cells with NCI-H460 lung cancer cells].
    Author: Zhang ZY, Wu JM, Pan HM.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 2006 Mar; 29(3):152-5. PubMed ID: 16677473.
    Abstract:
    OBJECTIVE: To study the antitumor effects induced by fusions of dendritic cells (DC) and NCI-H460 cells and compared with antigen pulsed DC. METHODS: (1) DC were induced from human monocytes and were fused with NCI-H460 cells. Three experimental groups were set up, including group fusions cell (FC), group pulsed cell (PC) and group T cell (TC), for which T cells activated by fusions, antigen pulsed DC, and non-activated T cells were used as the effector cells respectively. The killing activity of effector cells to NCI-H460 cells were evaluated by lactate dehydrogenase method. (2) NCI-H460 cells were injected subcutaneously to BALB/c nude mice. Then 18 cancer-bearing mice were divided randomly into 3 groups, group FC, group PC and group TC. Effector cells mentioned above were inoculated subcutaneously. The tumor size and tumor weight were assessed and compared. RESULTS: The killing rates to NCI-H460 cells of group FC, group PC and group TC were 43.54%, 26.57% and 3.25% respectively. Comparison of the killing activity of these 3 groups showed group FC > group PC > group TC (F = 5.47, P < 0.05). The tumor size of group FC was significantly smaller than that of group PC and group TC. The tumor weights of group FC, group PC and group TC were (1,129 +/- 123) mg, (1,709 +/- 160) mg and (3,344 +/- 288) mg respectively (F = 37.05, P < 0.01). CONCLUSION: Fusions of DC and NCI-H460 cells could induce powerful antitumor immunity, which was more effective than antigen pulsed DC.
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