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  • Title: Isolation of islets of Langerhans from the weanling pig.
    Author: Heald KA, Hail CA, Downing R.
    Journal: Diabetes Res; 1991 May; 17(1):7-12. PubMed ID: 1667860.
    Abstract:
    A simple method for the isolation of porcine islets of Langerhans is described. The technique, based on sequential collagenase digestion and density gradient separation through Percoll at unit gravity, produces islets which secrete insulin in response to glucose stimulation in static incubation, show bi-phasic release of insulin when perifused with 16 mM glucose, and can be maintained in culture for six weeks.
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