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  • Title: Column-switching high-performance liquid chromatographic analysis of fluvastatin in rat plasma by direct injection.
    Author: Um SY, Jung SH, Jung SJ, Kim JI, Chung SY, Lee HJ, Han SB, Choi SO.
    Journal: J Pharm Biomed Anal; 2006 Jun 16; 41(4):1458-62. PubMed ID: 16682159.
    Abstract:
    A column-switching high-performance liquid chromatographic (HPLC) method has been developed and validated for quantification of fluvastatin in rat plasma. Plasma samples were diluted with an equal volume of mobile phase, i.e. acetonitrile-5 mM potassium phosphate buffer (pH 6.8) (15:85, v/v), and the mixture was directly injected onto the HPLC system. The analyte was enriched in a pre-treatment column, while endogenous components were eluted to waste. The analyte was then back-flushed onto an analytical column and quantified with fluorescence detection (lambdaex=305 nm; lambdaem=390 nm). The standard curve for the drug was linear in the range 0.5-100 ng mL(-1) in rat plasma. The limit of quantitation for plasma was found to be 0.5 ng mL(-1). This method has been fully validated and shown to be specific, accurate and precise. The method is simple and rapid because of a minimized sample preparation and appears to be useful for the pharmacokinetic study of fluvastatin.
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