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  • Title: Surrogates for validation of electron beam irradiation of foods.
    Author: Rodriguez O, Castell-Perez ME, Ekpanyaskun N, Moreira RG, Castillo A.
    Journal: Int J Food Microbiol; 2006 Jul 15; 110(2):117-22. PubMed ID: 16690150.
    Abstract:
    The aim of this study was to identify a potential surrogate to describe the radiation sensitivity of the most common pathogens encountered in fruits. Three pathogens: Escherichia coli O157:H7 933, Listeria monocytogenes ATCC 51414, and Salmonella Poona, and five non-pathogens: E. coli K-12 MG1655, Listeria innocua Seeliger 1983 (NRRL B-33003 and NRRl B-33014), Enterobacter aerogenes, and Salmonella LT2 were inoculated (populations of 10(7)-10(9) CFU/ml) into model food systems (10% w/w gelatin) and exposed to doses up to 1.0 kGy using a 2 MeV Van der Graaf linear accelerator. The non-pathogen E. coli K-12 MG1655 was highly resistant to radiation (D(10)=0.88 kGy) in comparison to the other strains while L. monocytogenes was the more radiation-resistant pathogen (D(10)=1.09 kGy). Thus, E. coli K-12 MG1655 could be a suitable surrogate for e-beam studies with L. monocytogenes as the indicator pathogen. L. innocua strains were more radiation-sensitive (D(10)=0.66, 0.72 kGy) than their pathogenic counterpart. S. Poona and E. coli O157:H7 were even more radiation-sensitive (D(10)=0.38, 0.36 kGy, respectively). S. LT2 was the least radiation-resistant pathogen with D(10)=0.12 kGy. In a later study, the radiation resistance of the pathogens and the surrogate was evaluated when inoculated in a real food (i.e., fresh cantaloupe). The D(10) values obtained in this experiment were higher than those obtained with the model foods. However, the surrogate was still more radiation-resistant and could therefore be used to indicate decontamination of the target pathogens under electron beam irradiation.
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