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  • Title: Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR.
    Author: Cortez AL, Carvalho AC, Ikuno AA, Bürger KP, Vidal-Martins AM.
    Journal: Res Vet Sci; 2006 Dec; 81(3):340-4. PubMed ID: 16701750.
    Abstract:
    The present study was carried out to report the occurrence Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs. Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the invA, pefA, and sefA gene sequences from Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers.
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