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  • Title: [Determination of acetylharpagide in Ajuga decumbens by HPLC].
    Author: Yue DK, Ding GZ, Wang WH, Wang ZM, Li JR, Tong Y.
    Journal: Zhongguo Zhong Yao Za Zhi; 2006 Feb; 31(4):289-90. PubMed ID: 16706015.
    Abstract:
    OBJECTIVE: To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens. METHOD: The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm. RESULT: Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively. CONCLUSION: The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.
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