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Title: Improvement of the glaB promoter expressed in solid-state fermentation (SSF) of Aspergillus oryzae. Author: Ishida H, Hata Y, Kawato A, Abe Y. Journal: Biosci Biotechnol Biochem; 2006 May; 70(5):1181-7. PubMed ID: 16717420. Abstract: The glucoamylase-encoding gene (glaB) promoter should be very useful for recombinant protein production in solid-state fermentation (SSF) of Aspergillus oryzae. A 97-bp fragment containing the cis-element of the glaB promoter was inserted into the glaA promoter, which was little expressed in SSF. The chimeric promoter showed about a 24-fold increase in promoter activity in SSF. Eight copies of the 97-bp fragment were tandemly fused with the glaB promoter. The improved promoter showed about a 4.6-fold increase in promoter activity in SSF. The glaB gene was overexpressed under control of the improved glaB promoter in SSF. Recombinant glucoamylase production reached about 1524 mg/kg-broth for 2 d. The improved glaB promoter should be very useful for overproduction of a recombinant protein in SSF of A. oryzae.[Abstract] [Full Text] [Related] [New Search]