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  • Title: Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures.
    Author: Vinken M, Elaut G, Henkens T, Papeleu P, Snykers S, Vanhaecke T, Rogiers V.
    Journal: Methods Mol Biol; 2006; 320():247-54. PubMed ID: 16719396.
    Abstract:
    Mimicking the in vivo microenvironment is one of the current strategies to maintain liver-specific functionality in primary cultured hepatocytes for long periods. Freshly isolated hepatocytes entrapped in collagen gel type I (collagen gel immobilization culture) or sandwiched between two layers of hydrated collagen type I (collagen gel sandwich culture) are known to display liver-specific functions (e.g., biotransformation capacity) for more than 6 wk. We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture.
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