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  • Title: Phospholipase C catalyzed formation of sphinogomyelin--14C from lecithin and N-(-14C)-oleoyl-sphingosine.
    Author: Kanfer JN, Spielvogel CH.
    Journal: Lipids; 1975 Jul; 10(7):391-4. PubMed ID: 167259.
    Abstract:
    Commercial preparations of Clostreidium perfringens were incubated with phosphatidyl choline and N-1-(-14C) oleoylsphingosine. A radioactive product was formed which cochromatogramed with spingomyelin standard in three different solvent systems. Several other phospholipases and phosphatases were unable to catalyze this reaction. Neither choline, phosphoryl choline, cytidine diphosphate choline nor p-nitrophenyl phosphoryl choline were acitve donors. Sphingomyelin was only slightly active as a phosphoryl choline donor.
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