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  • Title: Routine immunophenotyping in acute leukemia: Role in lineage assignment and reassignment.
    Author: Qadir M, Barcos M, Stewart CC, Sait SN, Ford LA, Baer MR.
    Journal: Cytometry B Clin Cytom; 2006 Sep 15; 70(5):329-34. PubMed ID: 16739218.
    Abstract:
    Diagnostic evaluation of acute leukemia at Roswell Park Cancer Institute has routinely included immunophenotyping by multiparameter flow cytometry. In a retrospective analysis of 646 cases, morphology and cytochemistry established lineage in 612, but not in 34 (5%), of which 26, 5, and 3 were myeloid, undifferentiated, and lymphoid, respectively, based on immunophenotyping. In addition, immunophenotyping changed the lineage assigned based on morphology and cytochemistry in 11 cases (2%); 8 changed from lymphoid to myeloid, and 3 from myeloid to lymphoid. The data support routine inclusion of at least limited immunophenotyping in the diagnostic evaluation of acute leukemia.
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