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  • Title: Improving access to the control region and tRNA gene clusters of dipteran mitochondrial DNA.
    Author: Oliveira MT, Da Rosa AC, Azeredo-Espin AM, Lessinger AC.
    Journal: J Med Entomol; 2006 May; 43(3):636-9. PubMed ID: 16739428.
    Abstract:
    Despite the successful use of universal primers for amplifying insect mtDNA, specific regions remain difficult to recover and demand the use of taxon-specific primers. In this work, we describe a new set of primers for efficiently amplifying and sequencing the mtDNA control region and three tRNA gene clusters of dipterans of medical and veterinary importance, including Muscidae, Calliphoridae, and Oestridae species. These new primers were useful for investigating the nucleotide information and the structural organization of dipteran mtDNA.
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