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  • Title: Multivariate optimisation of the microwave-assisted extraction of oleuropein and related biophenols from olive leaves.
    Author: Japón-Luján R, Luque-Rodríguez JM, Luque de Castro MD.
    Journal: Anal Bioanal Chem; 2006 Jun; 385(4):753-9. PubMed ID: 16741775.
    Abstract:
    Microwave assistance is proposed for the first time in order to accelerate the extraction of biophenols from olive leaves. Under optimal working conditions, obtained using a multivariate methodology, complete extraction of the target analytes was achieved in 8 min. The extracts required no clean-up nor concentration prior to injection into a chromatograph-photodiode array detector assembly for individual separation-quantification. The optimal extractant (an 80:20 ethanol-water mixture) was also used in the development of a stirring-based extraction method which required around 24 h for complete extraction of the target compounds. These mixtures can be used as replacements for toxic extractants, with a view to exploiting olive leaves in order to obtain biophenols for human use.
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