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  • Title: Methods for the in vivo and in vitro analysis of [Het-s] prion infectivity.
    Author: Benkemoun L, Sabaté R, Malato L, Dos Reis S, Dalstra H, Saupe SJ, Maddelein ML.
    Journal: Methods; 2006 May; 39(1):61-7. PubMed ID: 16750391.
    Abstract:
    Prions have been described in mammals and fungi. The [Het-s] infectious genetic element of the filamentous fungus Podospora anserina is the prion form of the HET-s protein. This protein is involved in the control of a cell death reaction termed heterokaryon incompatibility. The infectious form of HET-s corresponds to a self-perpetuating amyloid. The purpose of the present paper is to describe the techniques that can be used to analyse [Het-s] prion propagation in vivo and HET-s amyloid aggregation in vitro. In addition, we report several methods that can be used to infect Podospora with recombinant HET-s amyloid.
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