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  • Title: [The effect of oncolytic adenovirus on human umbilical vein endothelial cell].
    Author: Ye ZM, Wang XH, Zhong J, Miao JC, Sheng WH, Xie YF, Wang JZ, Yang JC.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2006 May; 22(3):397-402. PubMed ID: 16755917.
    Abstract:
    The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
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