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  • Title: [HPLC-MSn analysis of trigonelline and its metabolites in rat urine].
    Author: Chen Y, Shen SL, Chen HX, Pan J, Han FM.
    Journal: Yao Xue Xue Bao; 2006 Mar; 41(3):216-20. PubMed ID: 16758990.
    Abstract:
    AIM: To establish a rapid and sensitive LC-MSn method for the identification of trigonelline and its main metabolites in rat urine. METHODS: After optimizing the detection conditions of LC-MSn chromatography and mass spectrometry using trigonelline, its ionization and cleavage in ESI-MS and ESI-MSn modes were summarized, then serving as the basis for the metabolite analysis of trigonelline in rat urine. The 0-48 h urine samples of rats were collected after iv 8 mg x kg(-1) trigonelline, then, the samples were purified through C18 solid-phase extraction cartridge. The purified samples were analyzed by LC-MSn. RESULTS: The structures of trigonelline metabolites were elucidated according to the changes of the molecular weights of the metabolites (deltaM) and their cleavage pattern in ESI-ITMSn. As a result, two phase I metabolites and the parent drug were identified existing in rat urine, and two phase II metabolites were identified. CONCLUSION: The LC-MSn method is rapid and high sensitive and specific, it is suitable for the identification of trigonelline and its metabolites in rat urine.
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