These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Detecting the DNA polymorphism of human complement component C8A by PCR-SSCP analysis].
    Author: Yang ZH, Zhang L, Zhou B, Wang C, Jia J.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2006 May; 37(3):471-3. PubMed ID: 16761437.
    Abstract:
    OBJECTIVE: The aim of this study is to establish a new method for detecting the DNA polymorphism of human complement component C8A. METHODS: Based on the point mutation (C-->A) of C8A gene in exon3,The genotypes of 98 unrelated individuals from Han population in Chengdu were studied by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP)analysis followed by DNA sequencing. RESULTS: The genotypes of C8A * AA, C8A * BB and C8A * AB were observed and the distribution of the genotypes frequencies of C8A in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. CONCLUSION: The method of PCR-SSCP is reliable, rapid, simple and cost-effective in detecting the DNA polymorphism of C8A, and it is valuable for further application in population genetic study and forensic science practice.
    [Abstract] [Full Text] [Related] [New Search]