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  • Title: Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays.
    Author: Semenyuk A, Ahnfelt M, Estmer Nilsson C, Yong Hao X, Földesi A, Kao YS, Chen HH, Kao WC, Peck K, Kwiatkowski M.
    Journal: Anal Biochem; 2006 Sep 01; 356(1):132-41. PubMed ID: 16769031.
    Abstract:
    A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.
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