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  • Title: Use of probes to detect virulence factor DNA sequences in Escherichia coli strains isolated from foods.
    Author: Franco BD, Gomes TA, Jakabi M, Marques LR.
    Journal: Int J Food Microbiol; 1991 Apr; 12(4):333-8. PubMed ID: 1677256.
    Abstract:
    Escherichia coli strains were isolated from 96 food samples (32 milks, 4 dairy products, 36 raw meats, 7 meat products, 7 sandwiches and 10 ready-to-eat meals). A total of 306 colonies was submitted to hybridization assays with DNA probes for the following virulence factors: heat-labile toxins (LT-I and LT-II), heat-stable toxins (ST-h and ST-p). Shiga-like toxins (SLT-I and SLT-II), adherence factor of enteropathogenic E. coli (EAF) and invasive factor (INV). Six colonies isolated from 4 food samples hybridized with the probes for LT-II (3 colonies isolated from a milk sample), SLT-I and SLT-II (1 colony isolated from raw bovine meat) or EAF (2 colonies isolated from two raw chicken meat samples).
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