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  • Title: Dicer-2 and R2D2 coordinately bind siRNA to promote assembly of the siRISC complexes.
    Author: Liu X, Jiang F, Kalidas S, Smith D, Liu Q.
    Journal: RNA; 2006 Aug; 12(8):1514-20. PubMed ID: 16775303.
    Abstract:
    In Drosophila melanogaster, the Dicer-2/R2D2 complex initiates RNA interference (RNAi) by processing long double-stranded RNA (dsRNA) into small interfering RNA (siRNA). Recent biochemical studies suggest that the Dcr-2/R2D2 complex also facilitates incorporation of siRNA into the RNA-induced silencing complex (siRISC). Here we present genetic evidence that R2D2 and Dcr-2 are both required for loading siRNA onto the siRISC complex. Consistent with this, only the Dcr-2/R2D2 complex, but neither Dcr-2 nor R2D2 alone, can efficiently interact with duplex siRNA. Furthermore, both dsRNA-binding domains of R2D2 are critical for binding to siRNA and promoting assembly of the siRISC complexes.
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