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  • Title: A synthetic peptide substrate for initiation factor-2 kinases.
    Author: Mellor H, Proud CG.
    Journal: Biochem Biophys Res Commun; 1991 Jul 31; 178(2):430-7. PubMed ID: 1677563.
    Abstract:
    A peptide P(45-56) corresponding to residues 45-56 (sequence: ILLSELSRRRIR) of eIF-2 alpha was synthesised. It was phosphorylated by both of the well characterised eIF-2 alpha kinases viz.; the heme-controlled repressor (HCR) and the double stranded RNA-dependent inhibitor (dsI). Of four other protein kinases tested only protein kinase C (PKC) phosphorylated P(45-56), with complete dependence on phosphatidylserine. Only the residue corresponding to serine-51 in eIF-2 alpha was phosphorylated by HCR, dsI or PKC. The phosphorylation of the peptide by dsI and the phosphorylation of eIF-2 alpha by dsI or HCR showed sigmoidal kinetics with respect to substrate concentration.
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