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  • Title: Selection of comparison criteria and experimental conditions to evaluate the kinetic performance of monolithic and packed-bed columns.
    Author: Eeltink S, Gzil P, Kok WT, Schoenmakers PJ, Desmet G.
    Journal: J Chromatogr A; 2006 Oct 13; 1130(1):108-14. PubMed ID: 16782110.
    Abstract:
    The present study concerns the problem of finding appropriate experimental conditions and comparison criteria to assess the kinetic performance of LC supports with different sizes or morphologies. A general procedure, based on evaluating each support for its own optimal mobile-phase composition, is proposed. The practical elaboration of the procedure is illustrated using the specific case of a capillary LC separation of a series of polycyclic aromatic test compounds employing silica-monolith capillary columns and capillary columns packed with 6-microm porous particles. To compare the systems for their ability to yield the fastest critical-pair separation, plate-height measurements are transformed into an effective plate number kinetic plot, i.e., a plot of the extrapolated retention time divided by the square of the extrapolated effective plate number (t(R)/N(eff)(2)) versus N(eff). This type of data representation provides a direct and universal basis to compare the kinetic performance of different LC supports and it corrects for differences in retention strength arising from different phase ratios.
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