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  • Title: Effects of storage time and temperature on urinary enzymes.
    Author: Matteucci E, Gregori G, Pellegrini L, Navalesi R, Giampietro O.
    Journal: Clin Chem; 1991 Aug; 37(8):1436-41. PubMed ID: 1678320.
    Abstract:
    We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 1.1.1.27; gamma-glutamyltransferase, GGT, EC 2.3.2.2; and alkaline phosphatase, AP, EC 3.1.3.1) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrations. Short-term (24 h) storage time at room temperature or 4 degree C does not affect urinary enzyme activity. A few days of freezing, at -20 or -70 degrees C, dramatically lowers LDH and AP values; GGT is partially preserved only at -70 degrees C, if the sample has been previously centrifuged. Urinary enzymes investigated in this study are extremely labile at low temperatures.
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