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  • Title: Production of high-content galacto-oligosaccharide by enzyme catalysis and fermentation with Kluyveromyces marxianus.
    Author: Cheng CC, Yu MC, Cheng TC, Sheu DC, Duan KJ, Tai WL.
    Journal: Biotechnol Lett; 2006 Jun; 28(11):793-7. PubMed ID: 16786243.
    Abstract:
    Of three beta-galactosidases from Aspergillus oryzae, Kluyveromyces lactis and Bacillus sp., used for the production of low-content galacto- oligosaccharides (GOS) from lactose, the latter produced the highest yield of trisaccharides and tetrasaccharides. GOS production was enhanced by mixing beta-galactosidase glucose oxidase. The low-content GOS syrups, produced either by beta-galactosidase alone or by the mixed enzyme system, were subjected to the fermentation by Kluyveromyces marxianus, whereby glucose, galactose, lactose and other disaccharides were depleted, resulting in up to 97% and 98% on a dry weight basis of high-content GOS with the yields of 31% and 32%, respectively.
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