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  • Title: Over-production of beta-carotene from metabolically engineered Escherichia coli.
    Author: Kim SW, Kim JB, Jung WH, Kim JH, Jung JK.
    Journal: Biotechnol Lett; 2006 Jun; 28(12):897-904. PubMed ID: 16786275.
    Abstract:
    To produce recombinant beta-carotene in vitro, synthetic operons encoding genes governing its biosynthesis were engineered into Escherichia coli. Constructs harboring these operons were introduced into either a high-copy or low-copy cloning vector. beta-Carotene production from these recombinant E. coli cells was either constitutive or inducible depending upon plasmid copy number. The most efficient beta-carotene production was with the low-copy based vector. The process was increased incrementally from a 5 l to a 50 l fermentor and finally into a 300 l fermentor. The maximal beta-carotene yields achieved using the 50 l and 300 l fermentor were 390 mg l(-1) and 240 mg l(-1), respectively, with overall productivities of 7.8 mg l(-1) h(-1) and 4.8 mg l(-1) h(-1).
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