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  • Title: [Role of Wnt/beta-catenin signaling transduction pathway in rat hepatocarcinogenesis].
    Author: Wang QM, Li X, Jia LQ, Yang KM, Zhou HY, Yang HJ.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2006 Jul; 22(4):454-7. PubMed ID: 16806007.
    Abstract:
    AIM: To investigate the role of Wnt/beta-catenin signaling transduction pathway in rat hepatocarcinogenesis. METHODS: The mRNAs of Wnt1, beta-catenin, APC, cyclin D1 and c-myc genes were amplified by using of semiquantitative reverse transcription polymerase chain reaction (RT-PCR) from normal rat livers, atypical hyperplasia livers and hepatoma tissues, respectively. Then the proteins expression of beta-catenin, APC and cyclin D1 was examined by immunohistochemical staining. RESULTS: In normal rat livers, the mRNAs of Wnt1, cyclin D1 and c-myc genes were not detected and only beta-catenin protein was observed to have low expression at cellular membrane. However, 14 weeks after cancer induction in atypical hyperplasia livers, beta-catenin protein and APC protein were accumulated in cytoplasm. Meanwhile, cyclin D1 protein was detected in cytoplasm and/or nucleus in some cells. 16 weeks after cancer induction in hepatoma tissues, the mRNAs and protein expression of beta-catenin, APC, cyclin D1 and c-myc genes were detected except Wnt1. CONCLUSION: The activation of Wnt/beta-catenin signaling transduction pathway might be one of the reasons for rat hepatocarcinogenesis.
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