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  • Title: [Effect of IL-6 and TNF-alpha on Dengue virus infection of human dendritic cells].
    Author: Shi YJ, Jiang ZY, Zeng K.
    Journal: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2006 Jul; 22(4):469-71. PubMed ID: 16806011.
    Abstract:
    AIM: To study the effect of IL-6 and TNF-alpha on Dengue virus (DV) infection of human dendritic cells (DCs). METHODS: Monocytes isolated from healthy human peripheral blood were incubated in medium with GM-CSF and IL-4 for 7-10 days, and then were collected and identified by transmission electron microscope, immunohistochemistry and lymphocytes stimulatory ability assay. DCs infected with DV type II (DV(2)) were incubated with IL-6, TNF-alpha at high, medium and low concentration. The supernatants were collected at 6 h, 24 h, 48 h, 72 h and 96 h. The virus titers were determined by plaque assay and the number of DCs at 48 h postinfection was mensurated by MTT. RESULTS: IL-6 at medium or low concentration increased DV(2) replication in DCs, whereas TNF-alpha at high and medium concentration inhibited DV(2) replication in DCs. Cytokines had no effect on the number of normal DCs. CONCLUSION: IL-6 and TNF-alpha may play an important role in the pathogenicity and immunity of Dengue virus.
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