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  • Title: Building of an immunosensor: how can the composition and structure of the thiol attachment layer affect the immunosensor efficiency?
    Author: Briand E, Salmain M, Herry JM, Perrot H, Compère C, Pradier CM.
    Journal: Biosens Bioelectron; 2006 Sep 15; 22(3):440-8. PubMed ID: 16806887.
    Abstract:
    Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated.
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