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  • Title: [Molecular cloning and expression analysis of swamp eel (Monopterus albus) Hprt].
    Author: He Y, Shang X, Cheng HH, Zhou RJ.
    Journal: Yi Chuan; 2006 Jun; 28(6):677-82. PubMed ID: 16818429.
    Abstract:
    The enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) plays an important role in the purine salvage pathways. Full-length cDNA of swamp eel (Monopterus albus, the rice field eel) Hprt gene was obtained by the RACE method. The cDNA sequence was 1452 bp in length, which encoded a protein of 218 amino acids. Amino acid identities of Hprt between swamp eel and other vertebrates including human, mouse, chicken and zebrafish were more than 76.7%. Phylogenetic tree analysis based on the amino acid sequences showed that Hprt of swamp eel and zebrafish was in one clade. RT-PCR analysis showed ubiquitous expression pattern of swamp eel Hprt in adult tissues, suggesting that it has a constitutive function and is conserved during evolution.
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