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  • Title: Quantitative thin-layer chromatography of pyrimethamine and related diaminopyrimidines in body fluids and tissues.
    Author: DeAngelis RL, Simmons WS, Nichol CA.
    Journal: J Chromatogr; 1975 Mar 19; 106(1):41-9. PubMed ID: 168216.
    Abstract:
    Several 2,4-diaminopyrimidines which inhibit the enzyme dihydrofolate reductase are quantitated following extraction and separation on silica gel thin-layer chromatographic plates. These compounds are candidates for the treatment of brain tumors and meningeal leukemia, because they have the ability to cross the blood-brain barrier. The ultraviolet absorption of the pyrimidine ring at 275 nm is utilized to quantitate these compounds on thin-layer chromatographic plates with a scanning instrument. This method offers the advantages of speed, specificity, versatility and sensitivity, and has proven to be satisfactory for the measurement of as little as 10 ng/ml of these compounds in biological fluids.
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