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  • Title: Selective sample preparation with bioaffinity columns prepared by the sol-gel method.
    Author: Cichna-Markl M.
    Journal: J Chromatogr A; 2006 Aug 18; 1124(1-2):167-80. PubMed ID: 16842802.
    Abstract:
    Bioaffinity materials prepared by entrapping highly selective bioligands in the pores of a sol-gel glass offer unique advantages for the clean-up of complex sample matrices. The inclusion of sol-gel bioaffinity columns frequently allows the efficient elimination of interfering matrix components and enrichment of analytes in a simple step. After introducing the basic principles the paper reviews the methods for the production of protein-doped sol-gel materials and discusses the characteristics of sol-gel affinity columns by comparing their stability, selectivity, binding capacity and reusability. The potential of sample clean-up with sol-gel affinity columns is demonstrated giving applications in environmental, food and clinical analysis.
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