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  • Title: Derivation and characterization of hepatocytes from embryonic stem cells in vitro.
    Author: Ishizaka S, Ouji Y, Yoshikawa M, Nakatani K.
    Journal: Methods Mol Biol; 2006; 330():387-99. PubMed ID: 16846038.
    Abstract:
    From a therapeutic perspective on liver, the use of embryonic stem (ES) technology in the generation of a large number of high-functional hepatocytes developed from ES cells for cell transplantation is anticipated. We have explored a three-dimensional culture system in which hepatocytes differentiated from mouse ES cells by transfection with the hepatocyte nuclear factor-3beta possess high metabolic functions that can be maintained long term.
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