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  • Title: [Developing of a new multicolor-fluorescent labeled STR amplification kit].
    Author: Li L, Chen GH, Li CT, Liu Y, Lin Y, Que TZ, Zhao ZM, Wang YX.
    Journal: Fa Yi Xue Za Zhi; 2006 Apr; 22(2):111-6. PubMed ID: 16850595.
    Abstract:
    OBJECTIVE: To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253). METHODS: By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci. RESULTS: Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit. CONCLUSION: The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.
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