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  • Title: [Study on tissue culture and rapid propagation of Atractylodes macrocephala].
    Author: Zhu YQ, Xia GH, Fang HG, Fu SH, He FJ.
    Journal: Zhong Yao Cai; 2006 Mar; 29(3):212-3. PubMed ID: 16850713.
    Abstract:
    A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.
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