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  • Title: Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules.
    Author: Jessel D, Hudson J, Landau T, Tenen D, Livingston DM.
    Journal: Proc Natl Acad Sci U S A; 1975 May; 72(5):1960-4. PubMed ID: 168585.
    Abstract:
    Mixing chromatographic fractions containing simian virus 40 (SV40) T antigen with SV40 [3H]-DNA I (double-stranded, circular, supercoiled) results in the conversion of the nucleic acid to a form that will bind to a nitrocellulose filter. Unlabeled SV40 DNA I successfully competes with this reaction. Under the conditions employed, the antigen-containing fractions bind a variety of circular, viral DNA molecules. Chromatography of the antigen in three systems reveals that the T immunoreactivity migrates with DNA binding activity. In a kinetic heat inactivation experiment, the antigenic reactivity disappears simultaneously with the DNA binding activity. The data indicate the presence of a discernible DNA binding activity in fractions containing T antigen and suggest that the T antigen is the DNA binding protein being measured.
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