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  • Title: Genetically engineered poly-gamma-glutamate producer from Bacillus subtilis ISW1214.
    Author: Ashiuchi M, Shimanouchi K, Horiuchi T, Kamei T, Misono H.
    Journal: Biosci Biotechnol Biochem; 2006 Jul; 70(7):1794-7. PubMed ID: 16861819.
    Abstract:
    The pgsBCA-gene disruptant from Bacillus subtilis ISW1214, i.e., MA41, does not produce poly-gamma-glutamate (PGA). We newly constructed an MA41 recombinant bearing the plasmid-borne PGA synthetic system, in which PGA production was strictly controlled by the use of xylose. Unlike the parent strain, ISW1214, the genetically engineered strain produced abundant PGA in both L-glutamate-rich and D-glutamate-rich media.
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