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  • Title: Molecular diagnosis of Nocardia farcinica from a cerebral abscess.
    Author: Tatti KM, Shieh WJ, Phillips S, Augenbraun M, Rao C, Zaki SR.
    Journal: Hum Pathol; 2006 Aug; 37(8):1117-21. PubMed ID: 16867876.
    Abstract:
    Histopathology and special stains of a brain biopsy specimen from a 42-year-old man revealed numerous gram-positive bacilli arranged in branching filaments, suggesting Nocardia infection. Antibiotic therapy with trimethoprim-sulfamethoxazole markedly decreased the abscess size, and the patient improved. DNA was analyzed from formalin-fixed sections of the cerebral abscess by a 16S ribosomal DNA polymerase chain reaction assay demonstrating the presence of either Nocardia farcinica or N otitidiscaviarum. A species-specific polymerase chain reaction assay confirmed N farcinica as the etiologic agent.
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